LYTA MUTANTS

Verification of D39 and the IFD-ply and -lytA mutants. PCR using ...

Download scientific diagram | Verification of D39 and the IFD-ply and -lytA mutants. PCR using different primer sets specific to ply and lytA were used to confirm ...

lyta mutants

Dynamic capsule restructuring by the main pneumococcal autolysin ...

29 Feb 2019 ... (a) WT TIGR4 or the isogenic deletion mutant in the lytA gene (LytA) were treated with 4 μg ml−1 LL-37 in a capsule-shedding assay for 30 or ...

lyta mutants

Differences in virulence of pneumolysin and autolysin mutants ...

21 Feb 2014 ... In PCR result, TGR occurred later in IDM-ply and -lytA mutants cultured in non- supplemented medium (4–5 h) compared with those grown in ...

lyta mutants

Differences in virulence of pneumolysin and autolysin mutants ...

21 Feb 2014 ... In PCR result, TGR occurred later in IDM-ply and -lytA mutants cultured in non- supplemented medium (4-5 h) compared with those grown in ...

lyta mutants

Rational stabilization of the C-LytA affinity tag by protein engineering ...

7 Oct 2008 ... Wild-type and mutant C-LytA proteins were purified by affinity chromatography from the overproducing Escherichia coli strain RB791 harboring ...

lyta mutants

LytA, Major Autolysin of Streptococcus pneumoniae, Requires ...

9 Feb 2012 ... To investigate the requirements of LytA activation, we established a genetic system that enabled the insertion of various LytA mutants into the ...

lyta mutants

Capsular Expression in Streptococcus pneumoniae Negatively ...

isolate I95 yielded nonencapsulated mutants that had lost antibiotic tolerance and were lytic to ... Derived from R6 (transformant with point mutation in lytA) [15].

lyta mutants

The Autolytic Enzyme LytA of Streptococcus pneumoniae Is Not ...

The lytA mutant strains were grown without added antibiotics. The stability of the lytA mutation under these conditions was confirmed by plating on blood agar ...

lyta mutants

Distinct and Specific Role of NlpC/P60 Endopeptidases LytA and ...

12 Apr 2019 ... Knockout mutants of 9 out of the 12 predicted PGHs were successfully obtained ( Rolain et al., 2012). Two PGHs, Acm2 and LytA, were shown ...

lyta mutants

Quinolones with enhanced bactericidal activity induce autolysis in ...

25 May 2009 ... Time-kill studies using lytA mutants showed that DC-159a exhibited slower killing than that against the lytA-positive strains. On exposure to the ...

lyta mutants

Pleiotropic Effects of Cell Wall Amidase LytA on Streptococcus ...

17 Nov 2014 ... Isogenic D39 mutants with mutations in lytA, ply, pspC, or lytB were constructed by transformation, using standard protocols (18, 21, 27, 28), ...

lyta mutants

The Autolysin-Encoding Gene (lytA) of Streptococcus pneumoniae ...

In support of a role for lytA in virulence, isogenic lytA mutants have been found to be significantly less virulent than the parent strain in some animal models (1, 2) ...

lyta mutants

Key Role of Amino Acid Residues in the Dimerization and Catalytic ...

15 Jun 2007 ... LytA, the main autolysin of Streptococcus pneumoniae, was the first ... During the process of constructing and sequencing the lytA mutants, ...

lyta mutants

(IUCr) Full-length structure of the major autolysin LytA

disable zoom view article download ppt. Figure 5. Enzymatic activity assays of LytA mutants. (a) Turbidometric assays. S. pneumoniae cells were treated with 10  ...

lyta mutants

Identification of key peptidoglycan hydrolases for morphogenesis ...

15 Oct 2012 ... Characterization of mutant cell morphology, growth, and autolytic behavior, in a range of conditions showed that 4 PGHs (Acm2, Lys2, LytA, ...

lyta mutants

PSGL-1 on Leukocytes is a Critical Component of ... - PLOS Pathogens

(A) ELISA showing binding of different concentrations of PSGL-1 to S. pneumoniae D39 (gray circles) and its isogenic lytA mutant (black squares). (B) ELISA ...

lyta mutants

Quinolones with Enhanced Bactericidal Activity Induce Autolysis in ...

25 May 2009 ... Time-kill studies using lytA mutants showed that DC-159a exhibited slower killing than that against the lytA-positive strains. On exposure to the ...

lyta mutants

Capsular Expression in Streptococcus pneumoniae Negatively ...

purified LytA gave a lower lytic response in capsulated strains, compared with that in nonencapsulated mutants. Spontaneous autolysis in stationary phase also  ...

lyta mutants

Octamer-Binding Sequence Is a Key Element for the Autoregulation ...

Therefore, ORF50/Lyta is an initiator for lytic viral replication. Lukac et al. reported that the KSHV ORF50/Lyta mutant, ΔSTAD, which lacks a serine/threonine-rich ...

lyta mutants

The puzzle of zmpB and extensive chain formation, autolysis defect ...

7 Jul 2008 ... However, the majority of CbpA was found trapped in the cytoplasm of the zmpB mutant. LytA was also detected mostly in the cytoplasm.

lyta mutants

Biological role of the pneumococcal amidase

Cloning of the lytA gene in Streptococcus pneumoniae. Concepcion RONDA, JosC ... in obtaining mutants in the genes encoding these enzymes. Streptococcus ...

lyta mutants

Full-length structure of the major autolysin LytA

LytA is responsible for the autolysis of many Streptococcus species, including pathogens such as S. ... lytic activities of the LytA CBS mutants, the LytA amidase.

lyta mutants

Octamer-binding sequence is a key element for the autoregulation of ...

Mutations in the octamer-binding motif resulted in refractoriness of the ORF50/ Lyta promoter to transactivation by ORF50/Lyta, and Oct-1 expression enhanced  ...

lyta mutants

Bacterial autolysins trim cell surface peptidoglycan to prevent ...

1 Apr 2014 ... Specifically, we tested mutants constructed in NCTC8325-4 S. aureus strain ... The S. pneumoniae lytA null mutant strain was constructed in the ...

lyta mutants

on December 29, 2019 at Google Indexer http://aac.asm.org ...

13 Aug 2012 ... 35 be involved in this process, lytA deficient mutants were constructed. In the. 36 presence of antibiotics, loss of LytA was not associated to an ...

lyta mutants

Key Role of Amino Acid Residues in the Dimerization and Catalytic ...

16 Apr 2007 ... a Lower case letters indicate nucleotides introduced to construct appropriate restriction sites (these are underlined) or lytA mutations.

lyta mutants

Tyrosine phosphorylation enhances activity of pneumococcal ...

A phospho-mimetic (Y264E) mutation of the LytA phosphorylation site displayed similar phenotypes as well as an enhanced dimerization capacity. Similarly ...

lyta mutants

Programmed Death in Bacteria | Microbiology and Molecular ...

In S. pneumoniae, autolysin LytA is responsible for autolysis, and lytA mutants are resistant to killing by cell wall inhibitors like penicillin (95) (antibiotics that are  ...

lyta mutants

Pyruvate oxidase of Streptococcus pneumoniae contributes to ...

9 Nov 2019 ... For additional studies strains lacking the major cell wall amidase LytA (ΔlytA) were created as described below. The ∆lytA isogenic mutants of ...

lyta mutants

Molecular analysis of antibiotic tolerance in pneumococci

Since mutations in this locus do not result in tolerance to penicillin alone, there must be other, yet ... the lytA mutant, also fail to produce LytA and are tolerant.

lyta mutants

Structural and Functional Insights into Peptidoglycan Access for the ...

11 Feb 2014 ... The cytosolic N-acetylmuramoyl-l-alanine amidase LytA protein of Streptococcus ... Activities of LytA point mutants in the turbidity lysis assay.

lyta mutants

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The M1 mutant is an in-frame insertion-deletion mutant where the coding ... concentration of WT and lytA mutant bacteria added to reactions was tested over a ...

lyta mutants

Molecular Peculiarities of the lytA Gene Isolated from Clinical ...

The autolytic LytA amidase from 12 bile (deoxycholate)-insoluble streptococcal ... This mutant has suffered a G-to-A transition at nucleotide 722 of the lytA gene, ...

lyta mutants

Genetic mapping of the <italic>lytA</italic> and <italic>lytB</italic ...

Genetic mapping of the lytA and lytB loci of Escherichia coli, which are involved in penicillin ... ture resistance in any of the lytA mutant strains, indicating that alas  ...

lyta mutants

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29 Jul 2008 ... (lytA::cam and lytC::tet) have been used for transformation of the R6 strain in order to generate single and double dele- tion mutants: lytA, lytC, ...

lyta mutants

5CTV: Catalytic domain of LytA, the major autolysin of ... - RCSB PDB

15 Jun 2019 ... 5CTV: Catalytic domain of LytA, the major autolysin of Streptococcus pneumoniae, (C60A, H133A, C136A mutant) complexed with ...

lyta mutants

Site - lytA

Name: lytA; Type: Gene; Mnemonic: lytic (autolysis and phage lysis); Left End Point: ... Temperature-sensitive autolysis-defective mutants of Escherichia coli.

lyta mutants

SpxB Is a Suicide Gene of Streptococcus pneumoniae and Confers ...

While lytA mutants did not lyse at stationary phase, they died at a rate similar to that of the wild-type strain. Furthermore, we show that the death process induced  ...

lyta mutants

Inhibition of Autolysis by Lipase LipA in Streptococcus pneumoniae ...

Keywords: infection, LipA, LytA, sepsis, S. pneumoniae ... However, in the presence of serum, the lytA fold change in the Δ lipA mutant was approximately 2,  ...

lyta mutants

Antibiotic tolerance in pneumococci - ScienceDirect

In mutants obtained in vitro, signaling pathways have been affected that show either ... In a tolerance assay, a lytA mutant of R6 is tolerant to both penicillin ( more ...

lyta mutants

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